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| Registros recuperados: 158 | |
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| OKLANDER,LUCIANA INÉS; STEINBERG3,ELIANA RUTH; DOLORES MUDRY,MARTA. |
| Given their great variability, microsatellites or STRs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in blood and hair samples in order to analyze the levels of genomic conservation among a wide range of primates including: Lemur catta, Alouatta caraya, Ateles belzebuth, Ateles chamek, Pan troglodytes, Papio sp., and Homo sapiens. In all cases we obtained amplification products that exhibited similar size both in monkeys and human (oscillating between 126 and 176 bp), except in the lemur where the detected fragment presented a size of approximately 1000 bp. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Primates; Microsatellite markers; PCR amplification; Non-invasive sampling; Genomic conservation; Genetic variability. |
| Ano: 2012 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2012000100007 |
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| Mathioni,Sandra Marisa; Carvalho,; Brunelli,Kátia Regiane; Beló,André; Camargo,Luis Eduardo Aranha. |
| For many years, the gray leaf spot disease (GLS) caused by the fungus Cercospora zeae-maydis Tehon & Daniels, was not considered an important pathogen of maize (Zea mays, L.) in Brazil. However, the recent adoption of agronomical practices such as no-tillage and cultivation under central pivot irrigation systems increased the incidence and severity to the extent that GLS is now one of the most important diseases of maize. Isolates of C. zeae-maydis can be distinguished by two genetic groups (I and II) based on AFLP markers and on polymorphisms of the ITS and 5.8S rDNA regions. Until now, however, the biological implications of this distinction remain unclear. This study investigated whether isolates from the two genetic groups differ in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Disease resistance; Gray leaf spot; Genetic variability; Pathogenicity. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162006000600006 |
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| Ambiel, Ana Claudia; UNOESTE; Guaberto, Luciana Machado; UNOESTE; Vanderlei, Talita Marques; UNOESTE; Machado Neto, Nelson Barbosa; UNOESTE. |
| O propósito deste trabalho foi determinar a similaridade genética entre acessos de germoplasma e cultivares comerciais de três espécies de Brachiaria (inter e intraespecífica), por meio de marcadores RAPD. Sementes foram utilizadas como fonte de DNA. 10 “primers” decâmeros foram selecionados de 120 “primers” avaliados, produzindo 107 bandas polimórficas, as quais foram utilizadas para a análise de variância molecular (Amova), o coeficiente de similaridade de Jaccard e índices de fixação gênica. 24,40% da variabilidade genética total estão contidas entre as espécies e 75,60% dentro destas, com índice de fixação gênica (FST) 0,24. No dendrograma, houve a formação de dois ramos, um formado por P. maximum e B. arrecta e o outro subdividido em três: todas... |
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Palavras-chave: 5.02.01.03-4 Genética e Melhoramento Florestal Brachiaria; Marcadores moleculares; Variabilidade genética; Dendrograma; Índice de fixação gênica. Brachiaria; Molecular markers; Genetic variability; Dendrogram; Genetic fixation index. |
| Ano: 2008 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5298 |
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| Paiva, Suzana de; Universidade Estadual de Maringá; Zawadzki, Claudio Henrique; Universidade Estadual de Maringa; Ruvulo-Takasusuki, Maria Claudia Colla; Universidade Estadual de Maringá; Lapenta, Ana Silvia; Universidade Estadual de Maringá; Renesto, Erasmo; Universidade Estadual de Maringá. |
| Allozyme electrophoresis analysis were performed in four species of Hypostomus (Loricariidae), H. albopunctatus, H. hermanni, H. regani, e Hypostomus sp. 1/NUP 5612 from the Ivaí river, a tributary of the upper Paraná river. The study of 14 loci revealed diagnostic characters and exclusive alleles in a low frequency. The heterozygosity ranged from 0.000 in H. albopunctatus to 0.199 in H. hermanni, which was higher than the heterozygosity in other samples of Hypostomus in literature, as well as in other fish groups. Hypostomus albopunctatus and H. regani revealed higher similarity (I = 0.804), while H. hermanni and Hypostomus sp. 1/NUP 5612 showed the least genetic identity (I = 0.569). All samples were genetically distinguished, despite there were several... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 2.02.00.00-5 catfish; Heterozygosity; Neotropical fishes; Genetic variability; Siluriformes Genética. |
| Ano: 2013 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/16355 |
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| Zawadzki,Cláudio H.; Renesto,Erasmo; Peres,Maria Dolores; Paiva,Suzana. |
| Three Brazilian populations of the armored catfish Hypostomus regani (Ihering, 1905) were sampled, one from the Corumbá Reservoir in Goiás state, another from the Itaipu Reservoir in Paraná state and a third from the Manso Reservoir in Mato Grosso state. Allozyme electrophoresis was used to establish the genetic structure of the species, with the analysis of liver, heart and muscles tissues allowing the scoring of 25 loci from 14 enzymatic systems. Although no diagnostic loci were found, some exclusive rare alleles were recorded for the three populations. The genetically most similar populations were those from Corumbá and Itaipu, and the most distant were the populations from Manso and Corumbá. The allozyme data showed three structured populations... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allozymes; Genetic variability; Hypostomus regani; Loricariidae; Paraguay and Paraná Rivers. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400025 |
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| Díaz-Matallana,Marcela; Schuler-García,Ingrid; Ruiz-García,Manuel; Hodson de Jaramillo,Elizabeth. |
| Currently in Colombia, there are only records of morph-agronomic characterizations of Mangifera indica cvar. Hilacha; molecular studies on this mango variety have not been carried out. The aim of this work was to identify the genetic diversity of six populations of mango Hilacha by RAPDs markers, as a fundamental base for breeding programs, conservation and selection of promissory materials for the fruit industry at the national level. From 60 primers evaluated in the populations, five primers were selected and were launched in the six populations. Polymorphic bands of RAPDs were transformed into binary matrices, which were then processed with NTSYS-PC, POPGENE and TFPGA softwares. The overall genetic diversity, H T = 0.468 ± 0.0016, is very similar to the... |
| Tipo: Journal article |
Palavras-chave: Genetic variability; Mango Hilacha; Organoleptic quality; RAPDs. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300001 |
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| D'Ávila,Marícia F.; Garcia,Rosane N.; Loreto,Elgion L. S.; Valente,Vera Lúcia da S.. |
| Drosophila willistoni (Sturtevant, 1916) is a species of the willistoni group of Drosophila having wide distribution from the South of USA (Florida) and Mexico to the North of Argentina. It has been subject of many evolutionary studies within the group, due to its considerable ability to successfully occupy a wide range of environments and also because of its great genetic variability expressed by different markers. The D. willistoni 17A2 strain was collected in 1991 in the state of Rio Grande do Sul, Brazil (30°05'S, 51°39'W), and has been maintained since then at the Drosophila laboratory of UFRGS. Different to the other D. willistoni strains maintained in the laboratory, the 17A2 strain spontaneously produced mutant males white-like (white eyes) and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: D. willistoni; 17A2 strain; Temperature stress; Genetic variability; Hypermutability. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212008000300009 |
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| Silveira,Sheila Recepute; Ruas,Paulo Maurício; Ruas,Claudete de Fátima; Sera,Tumoru; Carvalho,Valdemar de Paula; Coelho,Alexandre Siqueira Guedes. |
| The RAPD technique associated with restriction digestion of genomic DNA was used to assess the genetic variability within and among nine populations of Coffea arabica, including six progenies belonging to the Sarchimor germplasm, the progeny PR 77054-40-10 (Catuaí Vermelho IAC 81 x Icatu), and two commercial cultivars (IAPAR 59 and Catuaí Vermelho IAC-81). These populations were evaluated using analysis of molecular variance (AMOVA), genetic similarity among progenies, and percentage of polymorphic loci. A total of 99 RAPD markers were evaluated of which 67 (67.67%) were polymorphic. AMOVA showed that 38.5% and 61.5% of the genetic variation was distributed among and within populations, respectively. The fixation index (F ST) of the genotypes was 0.385.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffee breeding; Genetic variability; Molecular variance; RAPD markers. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300018 |
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| Lupchinski Junior, Enio; UEM; Vargas, Lauro; UEM; Povh, Jayme Aparecido; UEM; Ribeiro, Ricardo Pereira; UEM; Mangolin, Claudete Aparecida; UEM; Barrero, Nelson Maurício Lopera; UEM. |
| Este trabalho teve como objetivo analisar, pela técnica RAPD, a variabilidade e a divergência genética de duas gerações da linhagem GIFT. Foram estimados parâmetros para os reprodutores (G0) e para a progênie (F1). A variabilidade genética foi determinada pela porcentagem de loci polimórficos e pelo índice de Shannon. As gerações apresentaram 69,6% de loci polimórficos (G0) e 60,0% de polimorfismo (F1). Os valores para o índice de Shannon foram de 0,367 para a geração G0 e de 0,317 para a F1. Os valores de divergência genética, calculados pelo teste de Mantel, foram de 0,213 para a G0 e 0,208 para a geração F1. Os resultados obtidos indicaram que houve perda da variabilidade genética da geração G0 para a F1. No entanto, um fato a ser destacado foi a alta... |
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Palavras-chave: 5.05.04.00-2 Reprodução Animal índice de Shannon; Gerações de cultivo; Divergência genética; Variabilidade genética. Shannon index; Cultivated generations; Genetic divergence; Genetic variability. |
| Ano: 2008 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/4708 |
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| Cunha,Elizângela Emídio; Euclydes,Ricardo Frederico; Lopes,Paulo Sávio; Torres,Robledo de Almeida; Carneiro,Paulo Luiz Souza. |
| The aim of this work was to investigate the short-term behavior of the genetic variability of quantitative traits simulated from models with additive and non-additive gene action in control and phenotypic selection populations. Both traits, one with low (h² = 0.10) and the other with high (h² = 0.60) heritability, were controlled by 600 biallelic loci. From a standard genome, it was obtained six genetic models which included the following: only the additive gene effects; complete and positive dominance for 25, 50, 75 and 100% of the loci; and positive overdominance for 50% of the loci. In the models with dominance deviation, the additive allelic effects were also included for 100% of the loci. Genetic variability was quantified from generation to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Closed population; Computer simulation; Dominance deviation; Genetic variability; Intralocus interaction. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000900013 |
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| Araújo,Lourismar M; Neves,Leonarda G; Sousa,Demétrio A; Zeviani,Walmes M; Silva,Lucinéia da R; Marostega,Thalita N. |
| ABSTRACT The genetic diversity of pepper genotypes is usually evaluated based on morphological and molecular descriptors. However, studies using biochemical descriptors of peppers are still incipient. Thus, the objective of this study was identify biochemical descriptors of Capsicum spp. with antioxidant action, most responsive to the selection of superior genotypes, and evaluate the correlation between concentrations of the parameters using principal components analysis. The experiment was conducted in the city of Cáceres, southwest Mato Grosso, Brazil. Seventy-eight pepper accessions from the Active Germplasm Bank of UNEMAT were planted in randomized block design. The harvested fruits were immersed in liquid nitrogen for two minutes, and the following... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Capsicum spp.; Main components; Genetic variability. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362019000200210 |
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| Lopes-da-Silva,Marcelo; Tonet,Gabriela E.L.; Vieira,Luiz G.E.. |
| The greenbug Schizaphis graminum (Rondani) is one of the most important cereals pests in the world. Within populations of this species, several biotypes, which are clones that share same virulence relationships with cultivated plants, can be distinguished. Molecular markers have been used to genetically characterize insect populations because they are fast and cost effective. In order to find RAPD markers to identify Brazilian S. graminum biotypes, nineteen clonal populations of three biotypes (B, C and E) from Brazil and three clonal populations from the U.S. were examined. Eighteen primers were used to analyze the material, but only six primers revealed polymorphisms and among those, three produced diagnostic band profiles that allowed biotype... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic variability; Molecular marker; Greenbug; Insect-plant relationship. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2004000100009 |
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| Coelho, Marcela; Gonçalves-Vidigal, Maria Celeste; Sousa, Lorenna Lopes de; Nunes, Maria Paula Barion Alves; Azevedo, Rafhael Felipin; Galván, Marta Zulema. |
| The present work aimed characterize isolates of C. lindemuthianum race 65 from different regions in Brazil by ITS sequencing. A total of 17 isolates of race 65, collected in the states of Mato Grosso, Minas Gerais, Paraná, Santa Catarina and São Paulo, were studied. Analysis of the sequences of isolates 8, 9, 12, 14 and 15 revealed the presence of two single nucleotide polymorphisms (SNPs) in the ITS1 region at the same positions. These isolates, when analyzed together with the sequence of isolate 17, revealed a SNP in the ITS2 region. The highest genetic dissimilarity, observed between isolates 11 and 3 and between isolates 11 and 10, was 0.772. In turn, isolates 7 and 2 were the most similar, with a value of 0.002 for genetic distance. The phylogenetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 5.01.00.00-9; 5.01.03.00-8 anthracnose; Genetic variability; Pathogens; Dissimilarity Melhoramento Vegetal. |
| Ano: 2016 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/30586 |
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| Registros recuperados: 158 | |
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